Identifying old blood one corpuscle at a time
One must wonder if there is some applicability to shroud studies. An interesting paper was published online, yesterday, May 2, 2012, by the Journal of the Royal Society: Interface, entitled Preservation of 5300 year old red blood cells in the Iceman by Marek Janko, Robert W. Stark and Albert Zink. It is not behind a pay wall. I noticed this:
One corpuscle with a structure likely to be a RBC [24] was found in the hand wound tissue of the Iceman (figure 1d), and two single corpuscles were detected within the arrowhead wound sample. Sample B furthermore showed an agglomeration of several randomly distributed particles (figure 1f ). The selected corpuscles exhibit a discoidal, concave surface with a diameter between 5.8 and 6.4 mm (figure 1d,e). The concave shape is typical for RBCs and arises during the early stages of development in the bone marrow when the cell nuclei are discarded, leaving behind an impression on the membrane.
James Owen writing in National Geographic Daily News puts it in simpler words I appreciate:
"There were no [blood] traces found, even when they opened some arteries [of the 5,300-year-old mummy], so it was thought maybe the blood had not preserved and had completely degraded, or that he lost too much blood because of the arrow injury" on his back, said team member Albert Zink, head of the Institute for Mummies and the Iceman in Bolzano, Italy.
For the new investigation, scientists traced Ötzi’s wound areas—the arrow injury and a cut on his right hand—with a pioneering nano-size probe.
Each minute movement of the probe was recorded with a laser, "so you get a three-dimensional image of the sample in a very tiny scale," Zink explained.
The scans revealed classic "doughnut shape" red blood cells, the team reported Wednesday in the journal Interface.
[ . . . ]
The new nanotechnology, allied with an atomic force microscope, also uncovered traces of fibrin, a blood-clotting agent—evidence that the Iceman suffered a mercifully quick demise.
Image is from National Geographic
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Is the Shroud real? Probably.
The Shroud of Turin may be the real burial cloth of Jesus. The carbon dating, once seemingly proving it was a medieval fake, is now widely thought of as suspect and meaningless. Even the famous Atheist Richard Dawkins admits it is controversial. Christopher Ramsey, the director of the Oxford Radiocarbon Laboratory, thinks more testing is needed. So do many other scientists and archeologists. This is because there are significant scientific and non-religious reasons to doubt the validity of the tests. Chemical analysis, all nicely peer-reviewed in scientific journals and subsequently confirmed by numerous chemists, shows that samples tested are chemically unlike the whole cloth. It was probably a mixture of older threads and newer threads woven into the cloth as part of a medieval repair. Recent robust statistical studies add weight to this theory. Philip Ball, the former physical science editor for Nature when the carbon dating results were published, recently wrote: “It’s fair to say that, despite the seemingly definitive tests in 1988, the status of the Shroud of Turin is murkier than ever.” If we wish to be scientific we must admit we do not know how old the cloth is. But if the newer thread is about half of what was tested – and some evidence suggests that – it is possible that the cloth is from the time of Christ.
No one has a good idea how front and back images of a crucified man came to be on the cloth. Yes, it is possible to create images that look similar. But no one has created images that match the chemistry, peculiar superficiality and profoundly mysterious three-dimensional information content of the images on the Shroud. Again, this is all published in peer-reviewed scientific journals.
We simply do not have enough reliable information to arrive at a scientifically rigorous conclusion. Years ago, as a skeptic of the Shroud, I came to realize that while I might believe it was a fake, I could not know so from the facts. Now, as someone who believes it is the real burial shroud of Jesus of Nazareth, I similarly realize that a leap of faith over unanswered questions is essential.
My name is Dan Porter. Please email me at DanielRobertPorter@gmail.com
The saga of the iceman is an interesting case study in molecular forensics.
There are some obvious differences: a fairly well-preserved body vs. a cloth that is thought to have once covered a body, but I think there is some applicability, particularly in regards to the methods and technological advances. Since the original STURP investigation in 1978, the field of nanotechnology has continued to grow, allowing studies to be conducted with greater sensitivity and much more detail.
For the Iceman, sufficient intact DNA was available from the iceman for full genomic sequencing. These studies showed he was O-type blood, Rh positive; also, some other interesting characteristics (carrier of lyme disease, lactose intolerant, and had brown eyes). The images of the preserved red blood cells, and their detail, are quite remarkable. These were performed on sections taken from punch biopsies of the wound area using atomic force microscopy. In addition to microscopic data, some Raman spectra studies were shown in the paper, indicating the presence of fibrin.
With the Shroud, one is dealing with bloodstains, not an actual wound or biopsy samples, but I think there’s applicability here. Baima-Bollone described what appeared to be red blood cells on bloodstained fibers of the Shroud using electron microscopy (Sindon Vol. 33 (1984); 9-14; also in the book, Sindon O No, Eds. Torino, IT (1990). Red blood cells have been reported previously in mummies using microscopy (Science 108, 303-304 (1973), Science 192, 374-375 (1976), and on prehistoric rock tools (Science 220, 1269-1270 (1983), although not with the clarity available in the Iceman studies.
In bloodstains, as blood dries red blood cells dehydrate, similar to a tire going flat. What is most likely being visualized in such samples are aged blood cells that have resealed with each other during the preparation procedure, representing reconstituted cells, not intact red blood cells that have survived over large periods of time. Because of their distinct biconcave, disc-like shape, red blood cells may be identified microscopically with relative confidence. The new Iceman studies help to take this to another level.